ORCA - Lightning
Features
• High resolution : 4608 x 2592 (12 Megapixels)
• High Speed: 121 frames / s (at full resolution)
• Read noise : 2.0 electrons rms (Standard Full Well Capacity mode)
• Pixel size : 5.5 μm X 5.5 μm
• Dynamic Range 17000:1 (High Full Well Capacity mode)
• Peak QE : 60 % (at 550 nm)
高速影像擷取
• 可達到 6 倍於 ORCA - Flash 4.0 V3 的速度，並且具有相似的 readout noise。
• light - sheet 顯微鏡要求影像快速的輸出，由於讀取速度提高的圖像導致捕獲時獲得的光子更少，因此在嘗試捕獲高 S / N 圖像時，有必要將相機的 readout noise 保持在較低水準。
• ORCA - Lightning 可以完成這項困難的任務。
High speed test of 3D imaging
• We took consecutive images and produced 3D imaging of a free-swimming Volvox.
• When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.
• With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.
Imaging conditions
• Microscope: ezDSLM (light - sheet microscope)
• Objective lens: 10×
• Excitation wavelength: 488 nm
• Fluorescence wavelength: red (autofluorescence)
• Sample: Volvox
• Observation target: Free-swimming Volvox
• Method of imaging: Acquisition of approximately 8 volume image (stereoscopic) per second
